《植物生理学报》 2009, 45(3): 253-257

红豆杉细胞稳定生产紫杉醇中的同步化协同诱导作用

李丽琴, 付春华, 赵春芳, 王圣, 余龙江*

华中科技大学生命科学与技术学院, 武汉 430074

通信作者：余龙江；E-mail: yulj@hust.edu.cn；Tel: 027-87792264

摘　要：

同步化协同诱导可以稳定提高曼地亚红豆杉细胞培养物中紫杉醇含量。细胞培养周期第8 d的低温同步化处理可促 使细胞达到最高同步化率(20.4%), 而茉莉酸甲酯(MJ)的协同诱导可提高紫杉醇产量, 使紫杉醇产量最高值达到54.7 mg•L-1。 在细胞生长周期第 8 d, 未经低温同步化的红豆杉细胞中的关键酶基因 DXR、HMGR、GGPPS 和 DBAT 的表达量在 MJ 诱 导 24 h 后均迅速下降, 但在低温同步化的细胞中紫杉醇表达量下降缓慢, 60 h 后仍维持较高的水平。低温同步化和 MJ 协 同诱导的红豆杉细胞中, 紫杉醇合成关键酶基因高效稳定的表达可能是引起紫杉醇产量稳定提高的原因之一。

关键词：同步化; MJ 诱导; 曼地亚红豆杉; 紫杉醇

收稿：2008-12-12   修定：2009-01-16

资助：国家自然科学基金(20776058)和2006 年教育部新世纪优秀人才支持计划(NCET-06-0646)。

Effects of Synchronization and Synergistic Induction on Taxol Content in Taxus Cells

LI Li-Qin, FU Chun-Hua, ZHAO Chun-Fang, WANG Sheng, YU Long-Jiang*

Department of Biological Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China

Corresponding author: YU Long-Jiang; E-mail: yulj@hust.edu.cn; Tel: 027-87792264

Abstract:

Synchronization and synergistic induction could increase taxol content in Taxus media cv. Hicksii cells steadily. Cold treatment could improve the synchronization rate of cultured cells, the highest synchronization rate reached 20.4% in the 8^th d of a cell cycle. In the cells with cold treatment in the 8^th d, the taxol content could be further increased by the induction of methyl jasmonate (MJ), and the maximal taxol content reached 54.7 mg·L^-1. In the 8^th d, the expression levels of DXR, HMGR, GGPPS and DBAT decreased rapidly after 24 h induced by MJ in cells untreated with cold. On the contrary, these genes expression decreased slowly in cells with cold treatment, and the genes expression maintained higher levels after 60 h induced by MJ. Altogether, in cells with cold synchronization treatment and MJ induction, the high and steady expression of taxol biosynthetic pathway key genes maybe one reason for the steady improvement of taxol content.

Key words: synchronization; MJ induction; Taxus media cv. Hicksii; taxol